CN219122494U

CN219122494U - Confocal scanning system

Info

Publication number: CN219122494U
Application number: CN202320081789.2U
Authority: CN
Inventors: 却井山; 王子昂; 吴松; 蒋登斌; 朱婷
Original assignee: Hangzhou Bona Photoelectric Co ltd
Current assignee: Hangzhou Bona Photoelectric Co ltd
Priority date: 2023-01-13
Filing date: 2023-01-13
Publication date: 2023-06-02
Anticipated expiration: 2033-01-13

Abstract

The utility model discloses a confocal scanning system which comprises a light source module, a scanning module, a fluorescence guiding module, a signal detection module and an image reconstruction module, wherein the light source module is connected with the scanning module; the light source module is used for providing collimated multicolor laser; the scanning module irradiates the sample with multicolor laser provided by the light source module to emit a fluorescence signal by the laser; the fluorescence guiding module is used for conducting the fluorescence signal returned from the scanning module to the signal detecting module and filtering the fluorescence signal; the signal detection module is used for carrying out spectral detection on the fluorescent signal and outputting an electric signal; the image reconstruction module is used for receiving the electric signal sent by the signal detection module and restoring the image of the sample according to the electric signal. The utility model has the advantages of simple debugging operation and capability of meeting the requirements of most customers.

Description

Confocal scanning system

Technical Field

The utility model relates to the field of optical scanning detection imaging, in particular to a confocal scanning system.

Background

Confocal microscopes adopting laser spot scanning mode are important instruments for observing cell scale structures in the fields of biology, life sciences and the like, and are required to be applied in many occasions.

With respect to the related art in the above, the inventors consider that there are the following drawbacks: the existing confocal microscope has complex and precise structure, high cost and price, and complex operation; however, the requirements of most users are not very high, and the main concern is to acquire image information with higher resolution in a short time, so that the existing confocal microscope has too strong functions for most users, complicated debugging operation and low cost performance.

Disclosure of Invention

In order to meet the actual demands of most users, the utility model provides a confocal scanning system.

The utility model provides a confocal scanning system which adopts the following technical scheme:

a confocal scanning system comprises a light source module, a scanning module, a fluorescence guiding module, a signal detection module and an image reconstruction module;

the light source module is used for providing collimated multicolor laser;

the scanning module irradiates the sample with multicolor laser provided by the light source module to emit a fluorescence signal by the laser; the scanning module comprises a first adjustable mirror bracket; the first mirror adjusting frame is provided with a first reflecting mirror, and the angle of the first reflecting mirror is adjusted through the first mirror adjusting frame;

the fluorescence guiding module is used for conducting the fluorescence signal returned from the scanning module to the signal detecting module and filtering the fluorescence signal; the fluorescent guiding module comprises a second adjusting mirror bracket and a third adjusting mirror bracket; the second adjusting mirror bracket is provided with a second reflecting mirror, and the angle of the second reflecting mirror is adjusted through the second adjusting mirror bracket; a third reflector is arranged on the third adjusting mirror bracket, and the angle of the third reflector is adjusted through the third adjusting mirror bracket;

the signal detection module is used for carrying out spectral detection on the fluorescent signal and outputting an electric signal; the signal detection module is provided with a fluorescence signal detection channel for detecting fluorescence signals and a DIC signal detection channel for detecting DIC signals;

the image reconstruction module is used for receiving the electric signal sent by the signal detection module and restoring the image of the sample according to the electric signal.

By adopting the technical scheme, the light source module generates multicolor laser and enters the scanning module to irradiate the sample, so that the sample is subjected to laser to emit a fluorescent signal, then the fluorescent signal returns to enter the fluorescent guiding module through the scanning module, the fluorescent guiding module filters the fluorescent signal, and the fluorescent signal finally enters the signal detection module; the signal detection module converts the optical signal into an electric signal, and then the electric signal enters the image reconstruction module to restore the sample image; the angle of the first reflecting mirror of the scanning module can be adjusted through the first adjusting mirror frame, and the angles of the second reflecting mirror and the third reflecting mirror of the fluorescence guiding module can be respectively adjusted through the second adjusting mirror frame and the third adjusting mirror frame; meanwhile, the DIC signal detection channel and the fluorescent signal detection channel of the signal detection module are switched for use; therefore, when the image information with higher resolution is obtained, the first reflecting mirror, the second reflecting mirror and the third reflecting mirror can be adjusted for debugging, and the signal detection module detects the DIC signal and the fluorescent signal, so that the debugging operation is simple, and the requirements of most customers are met.

Optionally, the light source module includes a polychromatic laser generator, a first multimode fiber, a first fiber optic connector, and a first collimating lens; one end of the first multimode optical fiber is connected with the multicolor laser generator, and the other end of the first multimode optical fiber is connected with the first optical fiber connector; the first optical fiber connector is connected to the side surface of the system; the first collimating lens achromates and collimates polychromatic laser light emitted from the first fiber optic splice into parallel light.

By adopting the technical scheme, the polychromatic laser generated by the polychromatic laser generator passes through the first multimode optical fiber and the first optical fiber connector and then is emitted to the first collimating lens, and the first collimating lens achromates the polychromatic laser and collimates the polychromatic laser into parallel light.

Optionally, the scanning module further comprises a multi-passband beam splitter, an orthogonal galvanometer module, a scanning lens group, a field lens and an objective lens; the polychromatic laser sequentially passes through the first reflecting mirror, the multi-passband beam splitter, the orthogonal vibrating mirror module, the scanning lens group, the field lens and the objective lens and finally irradiates on a sample surface of a focus of the objective lens, and a fluorescent signal is emitted from the sample by the laser; the fluorescent signal sequentially passes through the objective lens, the field lens and the orthogonal galvanometer module and finally passes through the multi-passband beam splitter to enter the fluorescent guide module.

By adopting the technical scheme, multicolor laser sequentially passes through the first reflecting mirror, the multi-passband beam splitter, the orthogonal vibrating mirror module, the scanning lens group, the field lens and the objective lens to finally irradiate on a sample surface of a focus of the objective lens, and the sample emits a fluorescent signal by the laser; and then the fluorescent signal sequentially passes through the objective lens, the field lens and the orthogonal galvanometer module and finally passes through the multi-passband beam splitting sheet to enter the fluorescent guide module.

Optionally, the orthogonal galvanometer module comprises two galvanometers forming ninety degrees with each other; the angle of the vibrating mirror is adjustable.

By adopting the technical scheme, the orthogonal vibrating mirror module completes turning of the optical signal, and the angle of the vibrating mirror is adjustable so that the application range of the orthogonal vibrating mirror module is wider.

Optionally, the fluorescence guiding module further comprises a tele collecting lens, a variable pinhole, a second optical fiber connector and a second multimode optical fiber; the focal point of the long-focus collecting lens is positioned at the variable pinhole; the signal detection module comprises a third optical fiber connector; fluorescent signals sequentially pass through the second reflecting mirror, the third reflecting mirror, the long-focus collecting lens, the variable pinhole, the second optical fiber connector, the second multimode optical fiber and the third optical fiber connector.

Through adopting above-mentioned technical scheme, fluorescence signal passes through second speculum, third speculum, long burnt collecting lens in proper order, and fluorescence signal gathers the changeable pinhole department like this, and then fluorescence signal enters into signal detection module through second fiber connector, second multimode fiber and third fiber connector.

Optionally, the signal detection module comprises a terminal detection module and a switching module; the terminal detection module comprises a terminal photoelectric detector and a terminal bandpass filter; the switching module comprises a fourth reflecting mirror with an adjustable position; the fourth mirror has two positions and the two positions are a first position and a second position respectively; when the fourth mirror is in the first position, the fourth mirror is positioned between the terminal photodetector and the terminal bandpass filter and DIC signals from the DIC signal detection channels are reflected by the fourth mirror to the terminal photodetector; when the fourth reflecting mirror is at the second position, the fluorescent signal coming out of the fluorescent signal detection channel is emitted to a terminal photoelectric detector.

By adopting the technical scheme, the terminal detection module is controlled to process the DIC signal or the fluorescent signal by switching the fourth reflecting mirror between the first position and the second position.

Optionally, the fluorescent signal detection channel comprises a second collimating lens and a plurality of beam-splitting detection modules; the light-splitting detection module comprises a light-splitting sheet and a photoelectric detector; the second collimating lens performs achromatism and collimation on the incident fluorescent signals into parallel light; the fluorescent signal passes through the second collimating lens and then reaches the forefront light-splitting detection module; and the light splitting sheet reflects part of fluorescent signals to the photoelectric detector, and the rest fluorescent signals reach the light splitting sheet of the next light splitting detection module.

By adopting the technical scheme, after the fluorescent signals are achromatized and collimated into parallel light by the second collimating lens, the parallel light is split by the light splitting sheets which are distributed front and back in sequence, and the photoelectric detector processes the light signals split by the light splitting sheets.

Optionally, a bandpass filter is disposed between the beam splitter and the photodetector.

By adopting the technical scheme, the bandpass filter improves the filtering effect and the signal to noise ratio.

Optionally, the DIC signal detection channel comprises a fourth fiber optic connector, a third collimating lens and a fifth reflecting mirror; the third collimating lens performs achromatism and collimation on the DIC signal emitted from the fourth optical fiber connector into parallel light; the DIC signal is directed to the fifth mirror through the third collimating lens.

By adopting the above technical scheme, the DIC signal is achromatized and collimated into parallel light by the third collimating lens, reaches the fifth reflecting mirror and is reflected by the fifth reflecting mirror.

In summary, the beneficial effects of the utility model are as follows: the debugging operation is simple, and the requirements of most customers are met.

Drawings

Fig. 1 is a schematic view of the optical path principle of the present utility model.

Fig. 2 is a schematic diagram of the optical path principle of the signal detection module 40 of the present utility model.

Reference numerals illustrate:

10. a light source module; 11. a polychromatic laser generator; 12. a first multimode optical fiber; 13. a first optical fiber splice; 14. a first collimating lens;

20. a scanning module; 21. a first mirror; 22. a multi-passband beam splitter; 23. an orthogonal vibrating mirror module; 24. a scanning lens group; 25. a field lens; 26. an objective lens;

30. a fluorescence guide module; 31. a second mirror; 32. a third mirror; 33. a tele collection lens; 34. a variable pinhole; 35. a second optical fiber splice; 36. a second multimode optical fiber;

40. a signal detection module; 41. a third fiber optic connector; 42. a second collimating lens; 43. a beam-splitting detection module; 431. a light splitting sheet; 432. a bandpass filter; 433. a photodetector; 44. a terminal detection module; 441. a terminal bandpass filter; 442. a terminal photodetector; 45. a fourth mirror; 46. a driving element; 47. a fourth optical fiber splice; 48. a third collimating lens; 49. a fifth reflecting mirror;

50. and an image reconstruction module.

Detailed Description

The utility model is described in further detail below with reference to fig. 1-2.

The application discloses a confocal scanning system, referring to fig. 1 and 2, comprising a light source module 10, a scanning module 20, a fluorescence guiding module 30, a signal detection module 40 and an image reconstruction module 50; the light source module 10 is used for providing collimated polychromatic laser light; the scanning module 20 irradiates the sample with the multicolor laser provided by the light source module 10 to emit a fluorescence signal; the fluorescence guide module 30 is used for conducting the fluorescence signal returned from the scanning module 20 to the signal detection module 40 and filtering the fluorescence signal; the signal detection module 40 is used for performing spectral detection on the fluorescent signal and outputting an electric signal; the image reconstruction module 50 is configured to receive the electrical signal sent by the signal detection module 40 and restore an image of the sample according to the electrical signal.

Referring to fig. 1, a light source module 10 includes a multi-color laser generator 11, a first multimode optical fiber 12, a first optical fiber joint 13, and a first collimating lens 14; one end of the first multimode optical fiber 12 is connected with the polychromatic laser generator 11, and the other end is connected with the first optical fiber connector 13; a first fiber optic connector 13 is attached to the system side; the first collimating lens 14 achromates and collimates the polychromatic laser light emitted from the first fiber optic connector 13 into parallel light.

Referring to fig. 1, the scanning module 20 includes a first tunable mirror holder, a multi-passband beam splitter 22, an orthogonal galvanometer module 23, a scanning lens group 24, a field lens 25, and an objective lens 26; the first mirror 21 is arranged on the first mirror adjusting frame, and the angle of the first mirror 21 is adjusted through the first mirror adjusting frame; the orthogonal galvanometer module 23 comprises two galvanometers which are ninety degrees from each other; the angle of the vibrating mirror is adjustable; the polychromatic laser sequentially passes through the first reflecting mirror 21, the multi-passband beam splitter 22, the orthogonal vibrating mirror module 23, the scanning lens group 24, the field lens 25 and the objective lens 26 to finally irradiate on the sample surface of the focus of the objective lens 26, and the sample emits fluorescent signals by the laser; the fluorescent signal passes through the objective lens 26, the field lens 25, the orthogonal galvanometer module 23 and finally enters the fluorescent guide module 30 through the multi-passband beam splitter 22.

Referring to fig. 1, the fluorescence guide module 30 includes a second adjusting mirror holder, a third adjusting mirror holder, a tele collecting lens 33, a variable pinhole 34, a second fiber optic connector 35, and a second multimode fiber 36; the focal point of the tele collection lens 33 is located at the variable pinhole 34; the second multimode optical fiber 36 is connected to the optical fiber end surface of the second optical fiber connector 35 and is infinitely close to the focal point of the tele collecting lens 33; the second adjusting mirror frame is provided with a second reflecting mirror 31, and the angle of the second reflecting mirror 31 is adjusted by the second adjusting mirror frame; the third adjusting mirror frame is provided with a third reflecting mirror 32, and the angle of the third reflecting mirror 32 is adjusted by the third adjusting mirror frame; the fluorescent signal passes through the second reflecting mirror 31, the third reflecting mirror 32, the tele collecting lens 33, the variable pinhole 34, the second optical fiber connector 35, and the second multimode optical fiber 36 in this order.

Referring to fig. 2, the signal detection module 40 includes a fluorescent signal detection channel for detecting a fluorescent signal, a DIC signal detection channel for detecting a DIC signal, a terminal detection module 44, and a switching module;

referring to fig. 2, the fluorescent signal detection channel includes a third optical fiber connector 41, a second collimating lens 42, and a plurality of spectral detection modules 43; the beam-splitting detection module 43 comprises a beam-splitting sheet 431, a band-pass filter 432 and a photoelectric detector 433; the band-pass filter 432 is positioned between the beam splitter 431 and the photodetector 433; the second collimator lens 42 performs achromatism and collimation of the fluorescent signal incident from the third optical fiber connector 41 into parallel light; the fluorescence signal passes through the second collimating lens 42 and then reaches the forefront spectroscopic detection module 43; the beam splitter 431 reflects part of the fluorescence signal to the band-pass filter 432 for filtering, and then reaches the photoelectric detector 433, and the rest of the fluorescence signal reaches the beam splitter 431 of the next beam splitter detection module 43;

referring to fig. 2, the dic signal detection channel comprises a fourth optical fiber connector 47, a third collimating lens 48 and a fifth reflecting mirror 49; the third collimator lens 48 performs achromatizing and collimation of the DIC signal incident from the fourth optical fiber connector 47 into parallel light; the DIC signal passes through the third collimator lens 48 and is directed to the fifth mirror 49;

referring to fig. 2, the terminal detection module 44 includes a terminal photodetector 442 and a terminal bandpass filter 441; the switching module comprises a fourth reflecting mirror 45 with adjustable position; the fourth mirror 45 is driven by a driving element 46, which driving element 46 may be a motor; the fourth mirror 45 has two positions and the two positions are the first position and the second position, respectively; when the fourth mirror 45 is in the first position, the fourth mirror 45 is positioned between the terminal photodetector 442 and the terminal bandpass filter 441 and the DIC signal reflected from the fifth mirror 49 is reflected by the fourth mirror 45 to the terminal photodetector 442; when the fourth reflecting mirror 45 is at the second position, the fluorescent signal passing through the rearmost beam splitter 431 is filtered by the terminal bandpass filter 441 and then is emitted to the terminal photodetector 442.

The working principle of the confocal scanning system is as follows:

the polychromatic laser generator 11 generates polychromatic laser light, the polychromatic laser light reaches the first collimating lens 14 along the first multimode optical fiber 12 and the first optical fiber connector 13, the first collimating lens 14 performs achromatism and collimation on the polychromatic laser light into parallel lines, the polychromatic laser light sequentially passes through the first reflecting mirror 21, the multi-passband beam splitter 22, the orthogonal galvanometer module 23, the scanning lens group 24, the field lens 25 and the objective lens 26, finally irradiates on the sample surface of the focus of the objective lens 26, and the sample is subjected to laser fluorescence signal; the fluorescence signal sequentially passes through the objective lens 26, the field lens 25, the orthogonal vibrating mirror module 23 and finally passes through the multi-passband beam splitter 22, then enters the signal detection module 40 through the second reflecting mirror 31, the third reflecting mirror 32, the long-focus collecting lens 33, the variable pinhole 34, the second optical fiber connector 35, the second multimode optical fiber 36 and the third optical fiber connector 41, and then reaches the beam splitter detection module 43 at the forefront side after passing through the second collimating lens 42; the beam splitter 431 reflects part of the fluorescence signal to the band-pass filter 432 for filtering, and then reaches the photoelectric detector 433, and the rest of the fluorescence signal reaches the beam splitter 431 of the next beam splitter detection module 43; thus, when the fourth reflecting mirror 45 is at the second position, the fluorescent signal passing through the rearmost beam splitter 431 is filtered by the terminal bandpass filter 441 and then is emitted to the terminal photodetector 442; the DIC signal enters the signal detecting module 40 from the fourth optical fiber connector 47, then the DIC signal is first achromatized and collimated into parallel light through the third collimating lens 48, then reflected through the fifth reflecting mirror 49, and the reflected DIC signal is reflected to the terminal photodetector 442 through the fourth reflecting mirror 45 at the first position; finally, the DIC electrical signals and the fluorescent electrical signals processed by the signal detection module 40 enter the image reconstruction module 50 for signal processing, image reconstruction and other steps to restore the internal structure and information of the sample.

The above embodiments are not intended to limit the scope of the present utility model, so: all equivalent changes in structure, shape and principle of the utility model should be covered in the scope of protection of the utility model.

Claims

1. A confocal scanning system, characterized by: the device comprises a light source module (10), a scanning module (20), a fluorescence guiding module (30), a signal detection module (40) and an image reconstruction module (50);

the light source module (10) is used for providing collimated multicolor laser;

the scanning module (20) irradiates a sample with polychromatic laser light provided by the light source module (10) to emit a fluorescence signal by the laser light; the scanning module (20) includes a first adjustable frame; a first reflector (21) is arranged on the first lens adjusting frame, and the angle of the first reflector (21) is adjusted through the first lens adjusting frame;

the fluorescence guide module (30) is used for conducting fluorescence signals returned from the scanning module (20) to the signal detection module (40) and filtering the fluorescence signals; the fluorescence guide module (30) comprises a second adjusting mirror bracket and a third adjusting mirror bracket; a second reflector (31) is arranged on the second adjusting mirror bracket, and the angle of the second reflector (31) is adjusted through the second adjusting mirror bracket; a third mirror (32) is mounted on the third adjusting mirror frame, and the angle of the third mirror (32) is adjusted by the third adjusting mirror frame;

the signal detection module (40) is used for carrying out spectral detection on the fluorescent signal and outputting an electric signal; the signal detection module (40) is provided with a fluorescence signal detection channel for detecting fluorescence signals and a DIC signal detection channel for detecting DIC signals;

the image reconstruction module (50) is used for receiving the electric signal sent by the signal detection module (40) and restoring the image of the sample according to the electric signal.

2. A confocal scanning system according to claim 1, wherein: the light source module (10) comprises a multicolor laser generator (11), a first multimode optical fiber (12), a first optical fiber connector (13) and a first collimating lens (14); one end of the first multimode optical fiber (12) is connected with the multicolor laser generator (11), and the other end of the first multimode optical fiber is connected with the first optical fiber connector (13); -said first optical fibre connector (13) is connected to the system side; the first collimating lens (14) achromates and collimates the polychromatic laser light emitted from the first optical fiber (13) into parallel light.

3. A confocal scanning system according to claim 1, wherein: the scanning module (20) further comprises a multi-passband beam splitter (22), an orthogonal galvanometer module (23), a scanning lens group (24), a field lens (25) and an objective lens (26); the polychromatic laser sequentially passes through the first reflecting mirror (21), the multi-passband beam splitter (22), the orthogonal vibrating mirror module (23), the scanning lens group (24), the field lens (25) and the objective lens (26) to finally irradiate on a sample surface of a focus of the objective lens (26), and the sample emits fluorescent signals by the laser; the fluorescent signal sequentially passes through the objective lens (26), the field lens (25), the orthogonal vibrating mirror module (23) and finally passes through the multi-passband beam splitter (22) to enter the fluorescent guide module (30).

4. A confocal scanning system according to claim 3 wherein: the orthogonal galvanometer module (23) comprises two galvanometers which are ninety degrees each other; the angle of the vibrating mirror is adjustable.

5. A confocal scanning system according to claim 1, wherein: the fluorescence guiding module (30) further comprises a long-focus collecting lens (33), a variable pinhole (34), a second optical fiber connector (35) and a second multimode optical fiber (36); the focal point of the tele collecting lens (33) is located at the variable pinhole (34); the signal detection module (40) comprises a third optical fiber connector (41); fluorescent signals pass through the second reflecting mirror (31), the third reflecting mirror (32), the long-focus collecting lens (33), the variable pinhole (34), the second optical fiber connector (35), the second multimode optical fiber (36) and the third optical fiber connector (41) in sequence.

6. A confocal scanning system according to claim 1, wherein: the signal detection module (40) comprises a terminal detection module (44) and a switching module; the terminal detection module (44) comprises a terminal photoelectric detector (442) and a terminal bandpass filter (441); the switching module comprises a fourth reflecting mirror (45) with an adjustable position; the fourth mirror (45) has two positions and the two positions are a first position and a second position, respectively; when the fourth mirror (45) is in the first position, the fourth mirror (45) is positioned between the terminal photodetector (442) and the terminal bandpass filter (441) and DIC signals coming out of the DIC signal detection channels are reflected by the fourth mirror (45) to the terminal photodetector (442); when the fourth reflecting mirror (45) is at the second position, the fluorescent signal coming out of the fluorescent signal detection channel is emitted to a terminal photoelectric detector (442).

7. A confocal scanning system according to claim 1, wherein: the fluorescent signal detection channel comprises a second collimating lens (42) and a plurality of light-splitting detection modules (43); the light-splitting detection module (43) comprises a light-splitting sheet (431) and a photoelectric detector (433); the second collimating lens (42) performs achromatism and collimation on the incident fluorescent signal into parallel light; the fluorescence signal reaches the forefront light-splitting detection module (43) after passing through the second collimating lens (42); the light-splitting sheet (431) reflects part of the fluorescent signals to the photoelectric detector (433), and the rest of the fluorescent signals reach the light-splitting sheet (431) of the next light-splitting detection module (43).

8. The confocal scanning system of claim 7, wherein: a bandpass filter (432) is arranged between the beam splitting sheet (431) and the photodetector (433).

9. A confocal scanning system according to claim 1, wherein: the DIC signal detection channel comprises a fourth optical fiber connector (47), a third collimating lens (48) and a fifth reflecting mirror (49); the third collimating lens (48) performs achromatism and collimation of the DIC signal incident from the fourth optical fiber (47) into parallel light; the DIC signal passes through the third collimating lens (48) and is directed to a fifth mirror (49).